pet30b expression vectors (Millipore)
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pet30b expression vectors
Pet30b Expression Vectors, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30b+vector/pet28a/pmc11166852-160-4-7
Average 90 stars, based on 1 article reviews
Pet30b Expression Vectors, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30b+vector/pet28a/pmc11166852-160-4-7
Average 90 stars, based on 1 article reviews
pet30b expression vectors - by Bioz Stars,
2026-09
90/100 stars
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Clone Assay:Article Title: The characterization of novel monomeric creatine kinases in the early branching Alveolata species, Perkinsus marinus: Implications for phosphagen kinase evolution. Article Snippet: The genome of the unicellular molluscan parasite Perkinsus marinus contains at least five genes coding for putative creatine kinases (CK), a phosphoryl transfer enzyme which plays a key role in cellular energy transactions.. Expression and kinetic analyses of three of the P. marinus CKs revealed them to be true CKs with catalytic properties in the range of typical metazoan CKs.. A sequence comparison of the P. marinus CKs with a range of CK dimers and other dimeric phosphoryl transfer enzymes in this family (phosphagen kinases) showed that the P. marinus CKs lacked some of the critical residues involved in dimer stabilization, a trait all previously characterized CKs share. Article Title: Biochemical and molecular insights on the bioactivity and binding interactions of Bacillus australimaris NJB19 L-asparaginase. Article Snippet: L-asparaginase, an antileukemic enzyme, is indispensable to the treatment of Acute Lymphoblastic Leukemia (ALL).. However, the intrinsic glutaminase activity entails various side effects to the patients; thus, an improved version of the enzyme lacking glutaminase activity would be a requisite for effective treatment management of ALL.. The present study highlights the biochemical and molecular characteristics of the recombinant glutaminasefree L-asparaginase from Bacillus australimaris NJB19 (BaAsp). Article Title: Protease protection assays show polypeptide movement into the SecY channel by power strokes of the SecA ATPase Article Snippet: The flow‐through was collected and subjected to anion exchange chromatography (HiTrap Q FF, GE Healthcare Life Sciences, Marlborough, Massachusetts) followed by size exclusion chromatography (Superdex 200 10/300GL, GE Healthcare Life Sciences) in buffer C (50 mM HEPES/KOH pH 7.5, 50 mM KCl). .. DNA encoding cysteine‐free D209N WB SecA N95 with a C‐terminal His‐6 tag was cloned into the Article Title: Mini-III RNase-based dual-color system for in vivo mRNA tracking Article Snippet: The mR3 genes from 12 bacterial strains were synthesized by GenScript (see Table S1 for protein sequences of their inactive forms). .. The coding sequence of each mR3 gene was cloned into Article Title: Protease protection assays show polypeptide movement into the SecY channel by power strokes of the SecA ATPase Article Snippet: Protein expression and purification SecA, including all non‐WB SecA mutants, was purified essentially as previously described (Bauer et al , 2014 ). .. Specifically, a construct encoding cysteine‐free SecA N95 (lacking the non‐essential C‐terminus (Matsuyama et al , 1990 )) with a C‐terminal His‐6 tag and 3C protease cleavage site was cloned into the Plasmid Preparation:Article Title: The characterization of novel monomeric creatine kinases in the early branching Alveolata species, Perkinsus marinus: Implications for phosphagen kinase evolution. Article Snippet: The genome of the unicellular molluscan parasite Perkinsus marinus contains at least five genes coding for putative creatine kinases (CK), a phosphoryl transfer enzyme which plays a key role in cellular energy transactions.. Expression and kinetic analyses of three of the P. marinus CKs revealed them to be true CKs with catalytic properties in the range of typical metazoan CKs.. A sequence comparison of the P. marinus CKs with a range of CK dimers and other dimeric phosphoryl transfer enzymes in this family (phosphagen kinases) showed that the P. marinus CKs lacked some of the critical residues involved in dimer stabilization, a trait all previously characterized CKs share. Article Title: Biochemical and molecular insights on the bioactivity and binding interactions of Bacillus australimaris NJB19 L-asparaginase. Article Snippet: L-asparaginase, an antileukemic enzyme, is indispensable to the treatment of Acute Lymphoblastic Leukemia (ALL).. However, the intrinsic glutaminase activity entails various side effects to the patients; thus, an improved version of the enzyme lacking glutaminase activity would be a requisite for effective treatment management of ALL.. The present study highlights the biochemical and molecular characteristics of the recombinant glutaminasefree L-asparaginase from Bacillus australimaris NJB19 (BaAsp). Article Title: Protease protection assays show polypeptide movement into the SecY channel by power strokes of the SecA ATPase Article Snippet: The flow‐through was collected and subjected to anion exchange chromatography (HiTrap Q FF, GE Healthcare Life Sciences, Marlborough, Massachusetts) followed by size exclusion chromatography (Superdex 200 10/300GL, GE Healthcare Life Sciences) in buffer C (50 mM HEPES/KOH pH 7.5, 50 mM KCl). .. DNA encoding cysteine‐free D209N WB SecA N95 with a C‐terminal His‐6 tag was cloned into the Article Title: Testing the conservation of the translational machinery over evolution in diverse environments: assaying Thermus thermophilus ribosomes and initiation factors in a coupled transcription-translation system from Escherichia coli Article Snippet: .. PCR amplification from genomic T.thermophilus DNA was carried out, and products were digested with NdeI and EcoRI and ligated into the similarly digested Article Title: Mini-III RNase-based dual-color system for in vivo mRNA tracking Article Snippet: The mR3 genes from 12 bacterial strains were synthesized by GenScript (see Table S1 for protein sequences of their inactive forms). .. The coding sequence of each mR3 gene was cloned into Article Title: Protease protection assays show polypeptide movement into the SecY channel by power strokes of the SecA ATPase Article Snippet: Protein expression and purification SecA, including all non‐WB SecA mutants, was purified essentially as previously described (Bauer et al , 2014 ). .. Specifically, a construct encoding cysteine‐free SecA N95 (lacking the non‐essential C‐terminus (Matsuyama et al , 1990 )) with a C‐terminal His‐6 tag and 3C protease cleavage site was cloned into the Article Title: A potential type-II L-asparaginase from marine isolate Bacillus australimaris NJB19: Statistical optimization, in silico analysis and structural modeling. Article Snippet: International Journal of Biological Macromolecules 174 (2021) 527–539 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i j b iomac A potential type-II L-asparaginase from marine isolate Bacillus australimaris NJB19: Statistical optimization, in silico analysis and structural modeling Namrata Chakravarty a, Priyanka b, Jyoti Singh a,1, R.P.. Singh a,⁎ a Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India b Department of Chemical Engineering, Shiv Nadar University, NH-91, Tehsil Dadri Gautam Buddha Nagar, Uttar Pradesh 201314, India ⁎ Corresponding author.. E-mail address: r.singh@bt.iitr.ac.in (R.P. Article Title: A Study on the Effect of Surface Lysine to Arginine Mutagenesis on Protein Stability and Structure Using Green Fluorescent Protein Article Snippet: The Ni-NTA affinity column HisTrapHP was supplied by GE healthcare (Sweden). .. The Cloning:Article Title: The characterization of novel monomeric creatine kinases in the early branching Alveolata species, Perkinsus marinus: Implications for phosphagen kinase evolution. Article Snippet: The genome of the unicellular molluscan parasite Perkinsus marinus contains at least five genes coding for putative creatine kinases (CK), a phosphoryl transfer enzyme which plays a key role in cellular energy transactions.. Expression and kinetic analyses of three of the P. marinus CKs revealed them to be true CKs with catalytic properties in the range of typical metazoan CKs.. A sequence comparison of the P. marinus CKs with a range of CK dimers and other dimeric phosphoryl transfer enzymes in this family (phosphagen kinases) showed that the P. marinus CKs lacked some of the critical residues involved in dimer stabilization, a trait all previously characterized CKs share. Western Blot:Article Title: Protease protection assays show polypeptide movement into the SecY channel by power strokes of the SecA ATPase Article Snippet: The flow‐through was collected and subjected to anion exchange chromatography (HiTrap Q FF, GE Healthcare Life Sciences, Marlborough, Massachusetts) followed by size exclusion chromatography (Superdex 200 10/300GL, GE Healthcare Life Sciences) in buffer C (50 mM HEPES/KOH pH 7.5, 50 mM KCl). .. DNA encoding cysteine‐free D209N WB SecA N95 with a C‐terminal His‐6 tag was cloned into the pET30b vector (EMD Millipore, Burlington, Massachusetts) and expressed in BL21(DE3) E. coli cells (New England Biolabs, Ipswich, Massachusetts) for 4 h at 37°C after induction at OD 600 0.8 with 1 mM IPTG. .. Cells were collected by centrifugation for 10 min at 4,000 × g , resuspended in buffer A, and lysed by two passes through an EmulsiFlex‐C3 instrument (Avestin, Ottawa, Canada) at 20,000 psi. Polymerase Chain Reaction:Article Title: Testing the conservation of the translational machinery over evolution in diverse environments: assaying Thermus thermophilus ribosomes and initiation factors in a coupled transcription-translation system from Escherichia coli Article Snippet: .. PCR amplification from genomic T.thermophilus DNA was carried out, and products were digested with NdeI and EcoRI and ligated into the similarly digested Article Title: A potential type-II L-asparaginase from marine isolate Bacillus australimaris NJB19: Statistical optimization, in silico analysis and structural modeling. Article Snippet: International Journal of Biological Macromolecules 174 (2021) 527–539 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i j b iomac A potential type-II L-asparaginase from marine isolate Bacillus australimaris NJB19: Statistical optimization, in silico analysis and structural modeling Namrata Chakravarty a, Priyanka b, Jyoti Singh a,1, R.P.. Singh a,⁎ a Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India b Department of Chemical Engineering, Shiv Nadar University, NH-91, Tehsil Dadri Gautam Buddha Nagar, Uttar Pradesh 201314, India ⁎ Corresponding author.. E-mail address: r.singh@bt.iitr.ac.in (R.P. Amplification:Article Title: Testing the conservation of the translational machinery over evolution in diverse environments: assaying Thermus thermophilus ribosomes and initiation factors in a coupled transcription-translation system from Escherichia coli Article Snippet: .. PCR amplification from genomic T.thermophilus DNA was carried out, and products were digested with NdeI and EcoRI and ligated into the similarly digested Article Title: A potential type-II L-asparaginase from marine isolate Bacillus australimaris NJB19: Statistical optimization, in silico analysis and structural modeling. Article Snippet: International Journal of Biological Macromolecules 174 (2021) 527–539 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i j b iomac A potential type-II L-asparaginase from marine isolate Bacillus australimaris NJB19: Statistical optimization, in silico analysis and structural modeling Namrata Chakravarty a, Priyanka b, Jyoti Singh a,1, R.P.. Singh a,⁎ a Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India b Department of Chemical Engineering, Shiv Nadar University, NH-91, Tehsil Dadri Gautam Buddha Nagar, Uttar Pradesh 201314, India ⁎ Corresponding author.. E-mail address: r.singh@bt.iitr.ac.in (R.P. Sequencing:Article Title: Mini-III RNase-based dual-color system for in vivo mRNA tracking Article Snippet: The mR3 genes from 12 bacterial strains were synthesized by GenScript (see Table S1 for protein sequences of their inactive forms). .. The coding sequence of each mR3 gene was cloned into FLAG-tag:Article Title: Mini-III RNase-based dual-color system for in vivo mRNA tracking Article Snippet: The mR3 genes from 12 bacterial strains were synthesized by GenScript (see Table S1 for protein sequences of their inactive forms). .. The coding sequence of each mR3 gene was cloned into Construct:Article Title: Protease protection assays show polypeptide movement into the SecY channel by power strokes of the SecA ATPase Article Snippet: Protein expression and purification SecA, including all non‐WB SecA mutants, was purified essentially as previously described (Bauer et al , 2014 ). .. Specifically, a construct encoding cysteine‐free SecA N95 (lacking the non‐essential C‐terminus (Matsuyama et al , 1990 )) with a C‐terminal His‐6 tag and 3C protease cleavage site was cloned into the Gel Purification:Article Title: A potential type-II L-asparaginase from marine isolate Bacillus australimaris NJB19: Statistical optimization, in silico analysis and structural modeling. Article Snippet: International Journal of Biological Macromolecules 174 (2021) 527–539 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i j b iomac A potential type-II L-asparaginase from marine isolate Bacillus australimaris NJB19: Statistical optimization, in silico analysis and structural modeling Namrata Chakravarty a, Priyanka b, Jyoti Singh a,1, R.P.. Singh a,⁎ a Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India b Department of Chemical Engineering, Shiv Nadar University, NH-91, Tehsil Dadri Gautam Buddha Nagar, Uttar Pradesh 201314, India ⁎ Corresponding author.. E-mail address: r.singh@bt.iitr.ac.in (R.P. |